Identification of barley cultivars using SDS-PAGE electrophoresis
نویسندگان
چکیده
منابع مشابه
Multiple SDS-PAGE on Vertical Electrophoresis Units.
INTRODUCTION SDS-polyacrylamide gel electrophoresis (SDS-PAGE) represents the second-dimension separation of two-dimensional (2D)-PAGE. Large-scale proteome analysis usually requires simultaneous electrophoresis of batches of second-dimension SDS-PAGE gels to maximize the reproducibility of 2D electrophoresis protein profiles. This requirement is most easily met using multiple, vertical second-...
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We have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-DE). This modified version of 2-DE is not only simpler and easier but also faster than all the currently available methods. In this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrop...
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In the present study, an alternative procedure for two-dimensional (2D) electrophoretic analysis in proteomic investigation of the most represented basic muscle water-soluble proteins is suggested. Our method consists of Acetic acid-Urea-Triton polyacrylamide gel (AUT-PAGE) analysis in the first dimension and standard sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE) in the second dimension...
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Ehab Mohamed Rabei Metwali 1, 2*, Michael Paul Fuller 3, Salah Mohamed Hassan Gowayed 1, 2, Omar Abdel-Hakeim Almaghrabi 1 and Yahia Yosef Mosleh 1, 4 1 Biological Science Department, Faculty of Science, North Jeddah, King Abdul Aziz University, P. O. Box number 11508, Jeddah 21463, Saudi Arabia. 2 Botany Department, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt. 3 Depart...
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Plasmodium falciparum causes fatal cerebral malaria and more knowledge is required on its proteome. Proteomic studies require the adaptation of P. falciparum field isolates to continuous in vitro cultivation. The objective of this study is to identify and characterize some of the proteins secreted by P. falciparum trophozoites in the host cell using in vitro conditions. For this purpose, propag...
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ژورنال
عنوان ژورنال: Agricultural and Food Science
سال: 1992
ISSN: 1795-1895,1459-6067
DOI: 10.23986/afsci.72430